INNotope offers various 13C, 15N and 2H labeled RNA/DNA phosphoramidites for chemical RNA and DNA production using standard DNA/RNA synthesizers. The nucleic acids produced via this methods can be used for academic research in NMR or MS applications.

INNotope also offers 13C, 15N and 2H labeled RNA triphosphates, which can be directly used in RNA in vitro transcription.

RNA phosphoramidites
2′-O-TBS 5′-O-DMT 3′-CE phosphoramidite

RNA amidites with 13C label in nucleobase

[5-D, 6-13C]-uridine
pack size            list price
50 mg                 850 EUR
100 mg               1440 EUR
         250 mg               3400 EUR        

[5-D, 6-13C]-N4-Ac-cytidine
pack size            list price
50 mg                 850 EUR
100 mg               1440 EUR
250 mg               3400 EUR

[8-13C]-N6-Ac-adenosine
pack size            list price
50 mg                 850 EUR
100 mg               1440 EUR
250 mg               3400 EUR

[8-13C]-N2-Ac-guanosine
pack size            list price
50 mg                 850 EUR
100 mg               1440 EUR
250 mg               3400 EUR

RNA amidites with 15N label in nucleobase

[1,3-15N2]-uridine
pack size            list price
50 mg                 800 EUR
100 mg               1360 EUR
         250 mg               3200 EUR        

[1,3,4-15N3]-N4-Ac-cytidine
pack size            list price
50 mg                 800 EUR
100 mg               1360 EUR
250 mg               3200 EUR

[1-15N]-N6-Ac-adenosine
pack size            list price
50 mg                 800 EUR
100 mg               1360 EUR
250 mg               3200 EUR

[1-15N]-N2-Ac-guanosine
pack size            list price
50 mg                 900 EUR
100 mg               1530 EUR
250 mg               3600 EUR

 

modified DNA/RNA phosphoramidites
 5′-O-DMT (2′-TBS) 3′-CE phosphoramidite

methylated RNA amidites with 13C labels

m6A-13CH3
[N6-13CH3]-adenosine

pack size            list price
     50 mg             1100 EUR

m6A-13C2,8
[N6-CH3]-13C2,8-adenosine

pack size            list price
     50 mg              1100 EUR

m1A-13CH3
[N1-13CH3]-adenosine N6-2-chloroacetyl

pack size            list price
     50 mg             1100 EUR

m3C-13CH3
[N3-13CH3]-cytidine N4-acetyl

pack size            list price
     50 mg              1100 EUR

methylated DNA amidites with 13C labels

m5dC-13CH3
[5-13CH3]-2′-deoxycytidine N4-acetyl

pack size            list price
     50 mg              1100 EUR

m6dA-13C2,8
[N6-CH3]-13C2,8-2′-deoxyadenosine

pack size            list price
     50 mg              1100 EUR

DNA phosphoramidites
 5′-O-DMT 3′-CE phosphoramidite

DNA amidites with 13C label in nucleobase

[6-13C]-thymidine
pack size            list price
50 mg                 800 EUR
100 mg               1360 EUR
         250 mg               3200 EUR        

[5-D, 6-13C]-N4-Ac-2′-deoxycytidine
pack size            list price
50 mg                 800 EUR
100 mg               1360 EUR
250 mg               3200 EUR

[8-13C]-N6-Ac-2′-deoxyadenosine
pack size            list price
50 mg                 900 EUR
100 mg               1530 EUR
250 mg               3600 EUR

[8-13C]-N2-Ac-2′-deoxyguanosine
pack size            list price
50 mg                 900 EUR
100 mg               1530 EUR
250 mg               3600 EUR

DNA amidites with 15N label in nucleobase

[1,3-15N2]-thymidine
coming soon!

[1,3,4-15N3]-N4-Ac-2′-deoxycytidine
coming soon!

RNA triphosphates

RNA triphosphates are produced as triethylammonium salt form.

[5-D, 6-13C]-Uridine
coming soon!

[5-D, 6-13C]-Cytidine
coming soon!

[8-13C]-Adenosine
coming soon!

[8-13C]-Guanosine
coming soon!

Relevant References

  • Excited states of nucleic acids probed by proton relaxation dispersion NMR spectroscopy. Juen MA, Wunderlich CH, Nußbaumer F, Tollinger M, Kontaxis G, Konrat R, Hansen DF*, Kreutz C.*  Angew. Chem. Int. Ed. Engl. (2016);55(39):12008-12012. link
  • A Stably Protonated Adenine Nucleotide with a Highly Shifted pKa Value Stabilizes the Tertiary Structure of a GTP-Binding RNA Aptamer. Wolter AC, Weickhmann AK, Nasiri AH, Hantke K, Ohlenschläger O, Wunderlich CH, Kreutz C, Duchardt-Ferner E, Wöhnert J.* Angew. Chem. Int. Ed. Engl. (2017);56(1):401-404. link
  • Branch site bulge conformations in domain 6 determine functional sugar puckers in group II intron splicing. Plangger R, Juen M, Hoernes T, Nußbaumer F, Kremser J, Strebitzer E, Klingler D, Erharter K, Tollinger M, Erlacher M, Kreutz C.* Nucleic Acids Res. (2019); 47(21): 11430-11440. link
  • NMR chemical exchange measurements reveal that N6-methyladenosine slows RNA annealing.
    Shi H, Liu B, Nussbaumer F, Rangadurai A, Kreutz C*, Al-Hashimi HM.* J. Am. Chem. Soc. (2019); 141(51): 19988-19993. link